Physiological and biochemical effects of nickel on rainbow trout Oncorhynchus mykiss tissues Assessment of nuclear factor kappa B activation oxidative stress and histopathological changes
Yazarlar (4)
Prof. Dr. Ahmet Topal Atatürk Üniversitesi, Türkiye
Prof. Dr. Muhammed Atamanalp Atatürk Üniversitesi, Türkiye
Prof. Dr. Ertan Oruç Selçuk Üniversitesi, Türkiye
Doç. Dr. Hüseyin Serkan Erol Atatürk Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | CHEMOSPHERE | ||
| Dergi ISSN | 0045-6535 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 01-2017 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 01-01-2017 |
| Cilt / Sayı / Sayfa | 166 / 1 / 445–452 | DOI | 10.1016/j.chemosphere.2016.09.106 |
| Makale Linki | http://linkinghub.elsevier.com/retrieve/pii/S0045653516313078 | ||
| UAK Araştırma Alanları |
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| Özet |
| We investigated changes in nuclear factor kappa B (NFkB) activity, antioxidant responses and histopathological effects in the liver, gill and kidney tissues of rainbow trout exposed to nickel chloride (Ni). Two different concentrations (1 mg/L and 2 mg/L) were administrated to fish for 21 days. Tissues were taken from all fish for NFkB activity, histopathological examination and determination of superoxide dismutase (SOD), catalase (CAT) enzyme activity and of lipid peroxidation (LPO), and glutathione (GSH) levels. The findings of this study indicated that Ni exposure led to a significant increase in LPO indicating peroxidative damage and antioxidant enzymes SOD and CAT activity in tissues (p < 0.05), but 2 mg/Ni concentration caused a significant decrease in CAT activity in kidney tissues (p < 0.05). One of mechanism in the antioxidant defense system seems to be GSH, which increased in gill and kidney tissues of fish exposed to Ni (p < 0.05). NFkB immunopositivity was detected in all tissues. Ni exposure caused lamellar thickening, cellular infiltration in gill tissues, hydropic degeneration of hepatocytes in liver tissues, hyalinous accumulation within the glomeruli and tubular degeneration in kidney tissues. Our results suggested that Ni toxicity may disturb the biochemical and physiological functions of fish by causing changes in NFkB activity and oxidative and histopathological damage in the tissues of rainbow trout. This study can provide useful information for understanding of Ni-induced toxicity. |
| Anahtar Kelimeler |
| Fish | Heavy metal | Histopathology | NFkB | Nickel | Oxidative stress | Toxicity |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları |