Impact of Rho Kinase Inhibitor Hydroxyfasudil in Protamine Sulphate Induced Cystitis Rat Bladder
Yazarlar (10)
Yiğit Akın İzmir Katip Çelebi Üniversitesi, Türkiye
Prof. Dr. Aliseydi Bozkurt Erzincan Binali Yıldırım Üniversitesi, Türkiye
Doç. Dr. Hüseyin Serkan Erol Atatürk Üniversitesi, Türkiye
Prof. Dr. Mesut Bünyami Halıcı Atatürk Üniversitesi, Türkiye
Prof. Dr. Fikret Çelebi Atatürk Üniversitesi, Türkiye
Kübra Asena Terim Kapakin Atatürk Üniversitesi, Türkiye
Hakan Gülmez İzmir Demokrasi Üniversitesi, Türkiye
Mutlu Ateş Sağlık Bilimleri Üniversitesi, Türkiye
Taha Abdulkadir Çoban Erzincan Binali Yıldırım Üniversitesi, Türkiye
Barış Nuhoğlu İstanbul Yeni Yüzyıl Üniversitesi, Türkiye
| Makale Türü | Özgün Makale (SSCI, AHCI, SCI, SCI-Exp dergilerinde yayınlanan tam makale) | ||
| Dergi Adı | LUTS-LOWER URINARY TRACT SYMPTOMS | ||
| Dergi ISSN | 1757-5664 Wos Dergi Scopus Dergi | ||
| Dergi Tarandığı Indeksler | SCI-Expanded | ||
| Makale Dili | İngilizce | Basım Tarihi | 05-2015 |
| Kabul Tarihi | – | Yayınlanma Tarihi | 17-04-2014 |
| Cilt / Sayı / Sayfa | 7 / 2 / 108–114 | DOI | 10.1111/luts.12058 |
| Makale Linki | http://doi.wiley.com/10.1111/luts.12058 | ||
| UAK Araştırma Alanları |
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| Özet |
| Objectives: The objective of the present study was to evaluate anti-inflammatory effects of hydroxyfasudil in a protamine sulfate (PS) induced cystitis rat model. Additionally, we investigated prevention of bladder overactivity (BO), and tissue damage in these experiments. Methods: Animals were divided into four groups. In Groups 1 and 2, chemical induced cystitis model was created by administrating intravesical PS with PE50 catheter by the transurethral route. In Group 1, Rho-kinase inhibitor hydroxyfasudil was administered intaperitoneally, and in Group 2, subjects were administered a corresponding volume of saline in the same way. In Group 3, vehicle was administered intravesically and hydroxyfasudil was administrated intraperitoneally. Group 4 was a control Group, and the vehicle was administered intravesically and intraperitoneally. Micturition frequencies were recorded. Biochemical analyses were performed for oxidative stress, and pathological evaluations were investigated. In vitro contractions of bladder tissue strips were measured in tissue-bath. Results: There were significantly lower Lipid peroxidase levels and higher levels of Glutathione in Group 1 than Group 2 (P=0.016, P=0.001, respectively). There was generally more inflammation in Group 2 than the other groups as determined by microscopy. There were significantly higher frequencies of micturition, lower volume, and mean voided maximum urine output after PS administration in Groups 1 and 2. In vitro contraction responses of bladder strips to potassium chloride and acetylcholine were statistically higher in Group 2 than Groups 1 and 3. Conclusions: Significant reduction of inflammation by affecting the anti-oxidant defense systems was provided by hydroxyfasudil. Decreased in vitro responses to contractions of bladder smooth muscle strips were obtained. Hydroxyfasudil may be a potential new therapeutic option for inflammation and BO, in rat bladder. |
| Anahtar Kelimeler |
| Antioxidants | Bladder overactivity | Interstitial cystitis | Oxidative stress | Rho-kinase inhibitors |
BM Sürdürülebilir Kalkınma Amaçları
| Atıf Sayıları |