| Yazarlar |
Yiğit Akın
İzmir Katip Çelebi Üniversitesi, Türkiye |
|
Aliseydi Bozkurt
Erzincan Binali Yıldırım Üniversitesi, Türkiye |
Doç. Dr. Hüseyin Serkan EROL
Atatürk Üniversitesi, Türkiye |
|
Mesut Bünyami Halıcı
Atatürk Üniversitesi, Türkiye |
|
Fikret Çelebi
Atatürk Üniversitesi, Türkiye |
|
Kübra Asena Terim Kapakin
Atatürk Üniversitesi, Türkiye |
|
Hakan Gülmez
İzmir Demokrasi Üniversitesi, Türkiye |
|
Mutlu Ateş
Sağlık Bilimleri Üniversitesi, Türkiye |
|
Taha Abdulkadir Çoban
Erzincan Binali Yıldırım Üniversitesi, Türkiye |
|
Barış Nuhoğlu
İstanbul Yeni Yüzyıl Üniversitesi, Türkiye |
| Özet |
| Objectives: The objective of the present study was to evaluate anti-inflammatory effects of hydroxyfasudil in a protamine sulfate (PS) induced cystitis rat model. Additionally, we investigated prevention of bladder overactivity (BO), and tissue damage in these experiments. Methods: Animals were divided into four groups. In Groups 1 and 2, chemical induced cystitis model was created by administrating intravesical PS with PE50 catheter by the transurethral route. In Group 1, Rho-kinase inhibitor hydroxyfasudil was administered intaperitoneally, and in Group 2, subjects were administered a corresponding volume of saline in the same way. In Group 3, vehicle was administered intravesically and hydroxyfasudil was administrated intraperitoneally. Group 4 was a control Group, and the vehicle was administered intravesically and intraperitoneally. Micturition frequencies were recorded. Biochemical analyses were performed for oxidative stress, and pathological evaluations were investigated. In vitro contractions of bladder tissue strips were measured in tissue-bath. Results: There were significantly lower Lipid peroxidase levels and higher levels of Glutathione in Group 1 than Group 2 (P=0.016, P=0.001, respectively). There was generally more inflammation in Group 2 than the other groups as determined by microscopy. There were significantly higher frequencies of micturition, lower volume, and mean voided maximum urine output after PS administration in Groups 1 and 2. In vitro contraction responses of bladder strips to potassium chloride and acetylcholine were statistically higher in Group 2 than Groups 1 and 3. Conclusions: Significant reduction of inflammation by affecting the anti-oxidant defense systems was provided by hydroxyfasudil. Decreased in vitro responses to contractions of bladder smooth muscle strips were obtained. Hydroxyfasudil may be a potential new therapeutic option for inflammation and BO, in rat bladder. |
| Anahtar Kelimeler |
| Antioxidants | Bladder overactivity | Interstitial cystitis | Oxidative stress | Rho-kinase inhibitors |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | LUTS-Lower Urinary Tract Symptoms |
| Dergi ISSN | 1757-5664 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 05-2015 |
| Cilt No | 7 |
| Sayı | 2 |
| Sayfalar | 108 / 114 |
| Doi Numarası | 10.1111/luts.12058 |
| Makale Linki | http://doi.wiley.com/10.1111/luts.12058 |
| Atıf Sayıları | |
| WoS | 4 |
| SCOPUS | 4 |
| Google Scholar | 4 |
