| Yazarlar |
Fatime Geyikoğlu
Atatürk Üniversitesi, Türkiye |
|
Kübra Koç
Atatürk Üniversitesi, Türkiye |
Doç. Dr. Hüseyin Serkan EROL
Kastamonu Üniversitesi, Türkiye |
|
Suat Çolak
Erzincan Binali Yıldırım Üniversitesi, Türkiye |
|
Hafize Ayer
|
|
Shukrl Jama
|
|
Gizem Eser
Iğdır Üniversitesi, Türkiye |
|
Muhammed Bahaeddin Dortbudak
|
|
Yavuz Selim Sağlam
Atatürk Üniversitesi, Türkiye |
| Özet |
| Purpose: Ovarian ischemia-reperfusion (IR) damage continues to be a serious infertility problem. The oxidative stress plays central role in the development of IR injuries. Activation of antioxidants decreases IR injuries; however, the efficacy of antioxidant agents remains controversial. Unfortunately, there has been no evidence for medicinal use of boric acid (BA) and propolis (Prop) on ovarian IR injury on rats so far. This study will provide to reveal the potential applications of the Prop and BA in ovarian IR therapy. Methods: The Sprague–Dawley rats were randomized into five groups: I—control, II—IR, 3 h of ischemia and 3 h of reperfusion, III and IV—a signal dose of oral BA (7 mg/kg) and Prop (100 mg/kg) alone 1 h before induction of IR, V—Prop and BA together 1 h before induction of IR. SOD (superoxide dismutase), CAT (catalase), GSH (glutathione), MPO (myeloperoxidase), MDA (malondialdehyde), and IL-6 (interleukin-6) levels were quantified by ELISA and the TNF-α (tumor necrosis factor-α), 8-OHdG (8-hydroxylo-2′-deoxyguanosin) and Caspase-3 expressions were performed by immunohistochemical analyses. Results: BA and Prop pretreatment significantly reduced MPO, MDA, and IL-6 levels and pathologic score in IR rats, with no effects in control group. These agents used in therapy also decreased TNF-α, 8-OHdG and Caspase-3 protein expressions increased by IR. Furthermore, BA and Prop combination showed significant ameliorative effects on ovary injury caused by IR through acting as an antioxidant, anti-inflammatory and antiapoptotic agent. Conclusion: BA and Prop alone and especially in combination could be developed as therapeutic agents against ovary IR injury. |
| Anahtar Kelimeler |
| Boric acid | Inflammation | Ischemia-reperfusion | Ovary | Oxidative stress/antioxidant activity | Propolis |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | ARCHIVES OF GYNECOLOGY AND OBSTETRICS |
| Dergi ISSN | 0932-0067 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 10-2019 |
| Cilt No | 300 |
| Sayı | 5 |
| Sayfalar | 1405 / 1412 |
| Doi Numarası | 10.1007/s00404-019-05303-9 |
| Makale Linki | http://link.springer.com/10.1007/s00404-019-05303-9 |
| Atıf Sayıları | |
| WoS | 14 |
| SCOPUS | 13 |
| Google Scholar | 21 |
