| Yazarlar |
Harun Budak
Atatürk Üniversitesi, Türkiye |
|
Enver Fehim Koçpınar
Muş Alparslan Üniversitesi, Türkiye |
|
Nurdan Gönül
|
|
Hamid Ceylan
Atatürk Üniversitesi, Türkiye |
Doç. Dr. Hüseyin Serkan EROL
Atatürk Üniversitesi, Türkiye |
|
Orhan Erdoğan
Atatürk Üniversitesi, Türkiye |
| Özet |
| The trace elements such as iron are vital for various enzyme activities and for other cellular proteins, but iron toxicity causes the production of reactive oxygen species (ROS) that causes alterations in morphology and function of the nephron. The present study was designed to determine the effect of long-term iron overload on the renal antioxidant system and to determine any possible correlation between enzymatic and molecular levels. Our data showed that reduced glutathione (GSH) levels, which is a marker for oxidative stress, strikingly decreased with a long-term iron overload in rat kidney. While renal mRNA levels of glucose 6-phosphate dehydrogenase (G6pd), 6-phosphogluconate dehydrogenase (6pgd) and glutathione peroxidase (Gpx) were significantly affected in the presence of ferric iron, no changes were seen for glutathione reductase (Gsr) and glutathione S-transferases (Gst). While the iron affected the enzymatic activity of G6PD, GSR, GST, and GPX, it had no significant effect on 6PGD activity in the rat kidney. In conclusion, we reported here that the gene expression of G6pd, 6pgd, Gsr, Gpx, and Gst did not correlate to enzyme activity, and the actual effect of long-term iron overload on renal antioxidant system is observed at protein level. Furthermore, the influence of iron on the renal antioxidant system is different from its effect on the hepatic antioxidant system. © 2014 Elsevier Inc. |
| Anahtar Kelimeler |
| Antioxidant enzymes | Enzyme activity | Iron overload | Kidney | mRNA expression |
| Makale Türü | Özgün Makale |
| Makale Alt Türü | SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale |
| Dergi Adı | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY |
| Dergi ISSN | 1532-0456 |
| Dergi Tarandığı Indeksler | SCI-Expanded |
| Makale Dili | İngilizce |
| Basım Tarihi | 11-2014 |
| Cilt No | 166 |
| Sayı | 1 |
| Sayfalar | 44 / 50 |
| Doi Numarası | 10.1016/j.cbpc.2014.07.002 |
| Makale Linki | http://linkinghub.elsevier.com/retrieve/pii/S153204561400088X |
| Atıf Sayıları | |
| WoS | 28 |
| SCOPUS | 28 |
| Google Scholar | 40 |
