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Mucus from different fish species alleviates carrageenan-induced inflammatory paw edema in rats       
Yazarlar
Doç. Dr. Mustafa HİTİT
Kastamonu Üniversitesi, Türkiye
Orhan Çorum
Kastamonu Üniversitesi, Türkiye
Mehmet Özbek
Burdur Mehmet Akif Ersoy Üniversitesi, Türkiye
Kamil Üney
Selçuk Üniversitesi, Türkiye
Doç. Dr. Ertuğrul TERZİ
Kastamonu Üniversitesi, Türkiye
Gökhan Arslan
Atatürk Üniversitesi, Türkiye
Doç. Dr. Adem Yavuz SÖNMEZ
Kastamonu Üniversitesi, Türkiye
Özet
Objective: To determine the anti-inflammatory effects of mucus obtained from different fish species on the carrageenan-induced acute paw edema in rats. Methods: Forty-two rats were randomly divided into seven groups. Acute paw edema was induced by 0.1 mL of 1% carrageenan, and a single dose of diclofenac and lyophilized mucus (25 mg/kg) of rainbow trout, brook trout, European sea bass, and gilthead sea bream were administered to rats through gastric gavage 1 h before carrageenan treatment. Rat paws were measured before and 1-4 h after carrageenan treatment. The mRNA expressions of cytokines (TNF-α, IL-1β, IL-6, IL-10, and TGF-β), antioxidant markers (catalase and superoxide dismutase), and COX-2 were investigated using quantitative polymerase chain reaction. The histopathological changes were evaluated by hematoxylin and eosin staining. Results: The inhibition percentage of carrageenan-induced paw edema by different fish mucus ranged from 52.46% to 74.86% at 4 h. Histopathological evaluation showed that all fish mucus diminished carrageenan-induced edema and inflammatory cell infiltration. The upregulation of IL-1β mRNA induced by carrageenan was decreased by the mucus of rainbow trout and gilthead sea bream while an increase in the expression of IL-6 mRNA was reduced by the mucus of rainbow trout, brook trout, and gilthead sea bream. In addition, the mRNA expression of superoxide dismutase was higher in the rainbow trout mucus group than the carrageenan group. Conclusions: Mucus obtained from different fish species may have anti-inflammatory effects.
Anahtar Kelimeler
Carrageenan | Fish mucus | Inflammation | Rat paw edema
Makale Türü Özgün Makale
Makale Alt Türü SSCI, AHCI, SCI, SCI-Exp dergilerinde yayımlanan tam makale
Dergi Adı ASIAN PACIFIC JOURNAL OF TROPICAL BIOMEDICINE
Dergi ISSN 2221-1691
Dergi Tarandığı Indeksler SCI-Expanded
Makale Dili İngilizce
Basım Tarihi 10-2020
Cilt No 10
Sayı 10
Sayfalar 452 / 459
Doi Numarası 10.4103/2221-1691.290870
Makale Linki http://www.apjtb.org/text.asp?2020/10/10/452/290870